以琼脂半固体培基进行器官培养已证明能维持肿瘤组织较长期地体外生长,并且还可以连续传代(Wolff 和 Wolff;陆荣华等)。因此为研究肿瘤细胞生物学特性,抗癌药物筛选,体外的致癌作用,以及胚胎组织的分化与肿瘤组织的逆转等提供了较为理想的实验模型。但在实践中,尤其当研究某些化学物对器官组织的(不论是正常的,胚胎的,或是恶性的)作用时,往往需要用一系列浓度广为不同的药物;或药物的给予有时间顺序的连贯性(如研究致癌作用时先加起始剂,继之加促进剂);或同时使用两种或两种以上的药物。如此,用均一的琼脂培基甚为不便。再则,由于某些药物不宜加温,更使得应用琼脂培基具有局限 Organotyping with agar semi-solid culture has been shown to sustain longer in vitro growth of tumor tissue and is also capable of continuous passage (Wolff and Wolff; Lu Ronghua et al.). Therefore, it provides an ideal experimental model for the study of biological characteristics of tumor cells, screening of anticancer drugs, carcinogenesis in vitro, differentiation of embryonic tissues and reversal of tumor tissues. In practice, however, it is often necessary to use a series of widely differing concentrations of drugs, especially when studying the effects of certain chemicals on organ tissues (whether normal, embryonic, or malignant) Chronological consistency (eg, addition of starter when studying carcinogenesis) followed by the use of two or more drugs. Thus, with a uniform agar is very inconvenient. In addition, due to some drugs should not be warmed, but also makes the application of agar has limitations