目的通过构建Fndc5基因敲除小鼠,为后续的研究提供动物模型。方法运用TALEN技术在Fndc5基因FNIII domain中造成缺失突变,并通过测序进行基因型鉴定。通过配对建立稳定遗传系并在mRNA和DNA水平鉴定出生小鼠基因型;对不同年龄段出生小鼠进行体重、血糖分析;通过q PCR确定Fndc5在肾脏、肝脏、大脑、肌肉、心脏等组织中的表达情况。结果成功构建并鉴定得到4种不同的Fndc5基因敲除小鼠品系;不同年龄段出生小鼠体重、血糖未见显著性差异;确定Fndc5在肌肉、心脏等组织中高表达。结论本实验在国际上成功构建了Fndc5基因敲除小鼠,并进行了初步分析,为深入研究Fndc5基因在体内中的功能提供了动物模型。 Objective To construct animal model of Fndc5 knockout mice for further research. Methods TALEN technique was used to generate deletion mutations in FNIII domain of Fndc5 gene. Genotypes were identified by sequencing. Stable genetic lines were established by pairing and genotypes of the born mice were identified at the mRNA and DNA levels. Body weight and blood glucose analysis were performed on mice born at different ages. Fndc5 was identified by q-PCR in tissues of kidney, liver, brain, muscle and heart The expression of the situation. Results Four different strains of Fndc5 knockout mice were successfully constructed and identified. There was no significant difference in body weight and blood glucose among different age groups. It was confirmed that Fndc5 was highly expressed in muscle and heart tissues. Conclusions This experiment has successfully constructed Fndc5 knockout mice in the world and conducted preliminary analysis to provide an animal model for further study on the function of Fndc5 gene in vivo.