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目的:观察嗜酸乳杆菌LA14菌株耗尽培养上清液(spent culture supernatant,SCS)对兔离体肠平滑肌收缩的影响,并初步探讨其作用机制。方法:制备兔离体回肠标本,分别记录肠平滑肌的正常收缩频率和幅度作为给药前对照,然后按累积剂量分别加入嗜酸乳杆菌SCS、活菌菌液、SCS+活菌菌液。每次给药0.3ml,给药间隔6min,在每次给药4min后开始描记收缩曲线,记录时间2min。观察嗜酸乳杆菌各治疗组对离体肠平滑肌收缩的作用。另取肠段按毛果芸香碱、阿托品或SCS、再毛果芸香碱的顺序给药,观察嗜酸乳杆菌SCS对M型胆碱受体的作用。结果:按累积剂量连续给予SCS或SCS+活菌菌液0.6～1.5ml后,兔离体肠平滑肌收缩频率明显降低,与给药前比较差异有统计学意义(P<0.05或P<0.01),其余各点与给药前比较均无统计学差异。SCS、活菌菌液、SCS+活菌菌液在0.3～1.5ml剂量范围内,仅SCS+活菌菌液在给药达1.5ml时,兔离体肠平滑肌收缩幅度的降低与给药前比较有统计学差异(P<0.05),其余各点均无统计学差异。SCS或阿托品可明显对抗毛果芸香碱引起的兔离体肠平滑肌收缩幅度的增加(P<0.05或P<0.01),SCS还能使兔离体肠平滑肌收缩频率明显减少(P<0.01)。结论:嗜酸乳杆菌SCS可能通过阻断M型胆碱受体抑制兔离体肠平滑肌蠕动。
OBJECTIVE: To observe the effect of spent culture supernatant (SCS) of Lactobacillus acidophilus LA14 on contractility of isolated intestinal smooth muscle in rabbits and to explore its mechanism. Methods: Rabbit ileum specimens were prepared and the normal contraction frequency and amplitude of intestinal smooth muscle were recorded as pre-dose control. Then, Lactobacillus acidophilus SCS, viable bacteria solution and SCS + viable bacteria solution were added respectively according to cumulative dose. Each administration of 0.3ml, dosing interval 6min, 4min after each start tracing curve began to record the recording time 2min. To observe the role of Lactobacillus acidophilus treatment groups contraction of isolated intestinal smooth muscle. Another intestinal segment by pilocarpine, atropine or SCS, re-pilocarpine administered in order to observe the effect of Lactobacillus acidophilus SCS M-type choline receptors. Results: The contractile frequency of smooth muscle in isolated rabbit intestine was significantly decreased after continuous administration of 0.6-1.5 ml of SCS or SCS + viable bacteria solution at a cumulative dose, which was significantly different from that before administration (P <0.05 or P <0.01) The rest of the points compared with before administration no significant difference. SCS, live bacteria, SCS + live bacteria in the 0.3 ~ 1.5ml dose range, only SCS + live bacteria solution in up to 1.5ml, rabbit intestinal contraction muscle contraction amplitude compared with before administration there Statistical differences (P <0.05), the remaining points were not statistically different. SCS or atropine can obviously antagonize the increase of contraction amplitude of isolated smooth muscle in vitro (P <0.05 or P <0.01), and the contraction frequency of smooth muscle in isolated rabbit intestinal tissue decreased obviously (P <0.01). Conclusion: Lactobacillus acidophilus SCS may inhibit the in vitro intestinal smooth muscle motility of rabbits by blocking M-type cholinergic receptors.