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目的:研究环境污染物苯并(a)芘(Ba P)致人绒毛膜滋养层细胞HTR8-SV neo DNA氧化损伤的作用机制,探讨五味子乙素(Sch B)的可能保护作用。方法:本实验分为空白组、Ba P组、Sch B不同浓度组(0.1、0.5、2μmol/L),以HTR8-SV neo细胞为载体,构建Ba P氧化应激损伤模型,测定芳香烃受体(AHR)基因表达量,DNA的损伤情况,人X射线修复交差互补组1(XRCC1)和人多聚ADP核糖聚合酶(PARP1)基因及蛋白的表达水平。结果 :相比Ba P组,五味子乙素预处理组细胞AHR mRNA表达量及DNA损伤程度明显减轻(P<0.05),而细胞内XRCC1、PARP1 mRNA和蛋白的表达量逐渐增加(P<0.05)。结论:五味子乙素能预防Ba P致HTR8-SVneo细胞DNA的氧化损伤。
OBJECTIVE: To study the mechanism of oxidative damage of HTR8-SV neo DNA induced by environmental pollutants benzo (a) pyrene (Ba P) in human chorionic trophoblasts and to explore the possible protective effect of Schizandrin B (Sch B). Methods: The experiment was divided into blank group, Ba P group, Sch B different concentration groups (0.1, 0.5, 2 μmol / L), HTR8-SV neo cells as carrier, Ba P oxidative stress injury model was established, (AHR) gene expression, DNA damage, XRCC1 and PARP1 gene and protein expression in human X-ray. Results: Compared with Ba P group, the expression of AHR mRNA and DNA damage in cells treated with Schisandrin B was significantly reduced (P <0.05), and the expression of XRCC1 and PARP1 mRNA and protein in cells increased gradually (P <0.05) . Conclusion: Schisandrin B can prevent DNA damage induced by Ba P in HTR8-SVneo cells.