论文部分内容阅读
目的探讨环氧化酶2(COX2)在大鼠急性胰腺炎肺损伤中的作用。方法大鼠胰胆管逆行注射3.5%牛磺胆酸钠,制作大鼠重症急性胰腺炎并发肺损伤模型。将实验大鼠分为正常对照组、胰腺炎1、4、8、12、24小时组。分别对胰腺损伤、肺损伤程度进行病理评分,酶显色法测定血清脂肪酶水平,并测定肺组织湿/干比;逆转录聚合酶链反应技术检测各组鼠肺组织COX2的表达。结果造模后1小时,大鼠即出现较明显的胰腺损伤,伴有血清脂肪酶升高,随着时间的延长,胰腺损伤逐渐加重,以12小时为重。造模后1小时,肺损伤评分即有升高,但肺组织湿/干比无明显变化,此后,肺损伤程度逐渐增加,以24小时为重,光镜下主要表现为肺组织炎性细胞浸润、肺泡间隔增宽、肺泡腔内渗出液、肺泡腔塌陷等。正常大鼠肺组织内COX2mRNA呈低水平表达,造模4小时后,大鼠肺组织内COX2mRNA表达明显增加,至24小时达最高水平;大鼠肺组织内COX2mRNA表达与胰腺损伤程度、肺损伤程度成正相关。结论大鼠肺组织内COX2参与了胰腺炎肺损伤的过程,抑制肺组织内COX2的表达可能有助于胰腺炎和胰腺炎肺损伤的治疗。
Objective To investigate the role of cyclooxygenase 2 (COX2) in lung injury of acute pancreatitis in rats. Methods The rat pancreaticobiliary duct was injected retrogradely with 3.5% sodium taurocholate to make a rat model of severe acute pancreatitis complicated by lung injury. The experimental rats were divided into normal control group, pancreatic 1,4,8,12,24 hours group. The pathological score of pancreatic injury and lung injury were respectively measured. The level of serum lipase was measured by enzymatic chromogenic method. The wet / dry ratio of lung tissue was measured. The expression of COX2 was detected by reverse transcription polymerase chain reaction (RT-PCR). Results 1 hour after modeling, the rats showed obvious pancreatic injury, accompanied by elevated serum lipase, with the extension of time, pancreatic injury gradually increased to 12 hours as the weight. One hour after modeling, the lung injury score increased, but the wet / dry ratio of the lung tissue had no significant change. Thereafter, the degree of lung injury gradually increased to 24 hours as the weight, mainly under the light microscope lung inflammatory cells Infiltration, widened alveolar septa, alveolar exudate, alveolar collapse and so on. The expression of COX2mRNA in the lung tissue of normal rats was low at 4h after injection, and the expression of COX2mRNA in the lung tissue of rats increased significantly up to 24 hours after the model was established. The COX2mRNA expression in the lung tissue of rats was significantly correlated with the degree of pancreatic injury, Into a positive correlation. Conclusion COX2 is involved in the process of lung injury induced by pancreatitis in rats. Inhibition of COX2 expression in lung tissue may contribute to the treatment of pancreatitis and pancreatitis with lung injury.