维拉帕米(Verapamil,VR),又称异搏停,近年来已作为抗心绞痛、抗高血压及抗心律失常的药物为临床广泛应用。为探索VR治疗室上性快速型心律失常的机理和产生临床效应时对细胞内钙和膜钙泵的影响,我们用荧光法测定了血浆中VR浓度。 方法 一、试剂维拉帕米标准液(2.5mg/ml)芬兰产品。 二、仪器 日立650-60荧光分光光度计。 三、确立VR的最佳激发波长与发射波长标准VR溶于0.1 mol/L盐酸(外标准管),用荧光分光光度计扫描,其最佳激发波长为 Verapamil (Verapamil, also known as verapamil, in recent years has been used as anti-angina, antihypertensive and anti-arrhythmic drugs for clinical use. To explore the mechanism of VR in the treatment of supraventricular tachyarrhythmia and its effect on intracellular calcium and membrane calcium pumps in the generation of clinical effects, we measured the plasma VR concentration by fluorescence. Method one, the reagent verapamil standard solution (2.5mg / ml) Finnish product. Second, the instrument Hitachi 650-60 fluorescence spectrophotometer. Third, to establish the best VR excitation wavelength and emission wavelength standard VR dissolved in 0.1 mol / L hydrochloric acid (external standard tube), with a fluorescence spectrophotometer scan, the best excitation wavelength of