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本实验采用改良栓线法制备大鼠右侧大脑中动脉 (Middlecerebralartery ,MCA)缺血再灌注模型 ,腹腔注射阈下剂量 (35mg/kg·2d)戊四唑 (pentylenetetrazol,PTZ)制备慢性癫痫点燃模型。通过观察大鼠的行为 ,来检测其癫痫敏感性的改变。分别用硫堇染色、免疫细胞化学方法观察PTZ点燃脑缺血大鼠的相应脑区的神经病理学改变及脑内胶质原纤维酸性蛋白 (gliafibrillaryacidicprotein ,GFAP)免疫反应活性 (immunoreactivity,IR)的变化。结果显示 :脑缺血后大鼠癫痫敏感性明显增强 (P <0 0 5 )。右背侧海马CA1、CA3 区锥体细胞、额叶皮质神经元不同程度的脱失 (P <0 0 5 )并出现大量GFAP免疫反应阳性的星形胶质细胞 ,且细胞体积变大 ,突起变长变粗 ,免疫染色强度明显增强 (P <0 0 5 ) ,提示脑缺血大鼠癫痫敏感性增强 ,可能与海马及额叶皮质的神经元脱失及星形胶质细胞活化增生有关。
In this study, MCAO models of ischemia-reperfusion were established by modified plug method and peritoneal injection of subthreshold dose (35mg / kg · 2d) of pentylenetetrazol (PTZ) model. By observing the behavior of rats, to detect changes in the sensitivity of epilepsy. The changes of neuropathology and the changes of immunoreactivity (IR) of brain glial fibrillary acidic protein (gliafibrillary acid protein, GFAP) were observed by thionine staining and immunocytochemistry. . The results showed that the sensitivity of epilepsy in rats with cerebral ischemia increased significantly (P <0.05). In the right dorsal hippocampal CA1, CA3 pyramidal cells and frontal cortex neurons were lost to varying degrees (P <0 05) and a large number of GFAP immunoreactive positive astrocytes, and the cells become larger, the protrusions And became thicker and stronger in immunostaining (P <0 05), suggesting that the sensitivity of epilepsy increased in rats with cerebral ischemia, which may be related to the loss of neurons in hippocampus and frontal cortex and the activation and proliferation of astrocytes .