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As a representative drug for the treatment of severe community-acquired pneumonia and sepsis,Xuebijing(XBJ)injec-tion is also one of the recommended drugs for the prevention and treatment of coronavirus disease 2019(COVID-19),but its treatment mechanism for COVID-19 is still unclear.Therefore,this study aims to explore the potential mechanism of XBJ injection in the treat-ment of COVID-19 employing network pharmacology and molecular docking methods.The corresponding target genes of 45 main active ingredients in XBJ injection and COVID-19 were obtained by using multiple database retrieval and literature mining.102 over-lapping targets of them were screened as the core targets for analysis.Then built the PPI network,TCM-compound-target-disease,and disease-target-pathway networks with the help of Cytoscape 3.6.1 software.After that,utilized DAVID to perform gene ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to predict the ac-tion mechanism of overlapping targets.Finally,by applying molecular docking technology,all compounds were docked with COVID-19 3 CL protease(3CLpro),spike protein(S protein),and angiotensin-converting enzyme Ⅱ(ACE2).The results indicated that quercet-in,luteolin,apigenin and other compounds in XBJ injection could affect TNF,MAPK1,IL6 and other overlapping targets.Meanwhile,anhydrosafflor yellow B(AHSYB),salvianolic acid B(SAB),and rutin could combine with COVID-19 crucial proteins,and then played the role of anti-inflammatory,antiviral and immune response to treat COVID-19.This study revealed the multiple active com-ponents,multiple targets,and multiple pathways of XBJ injection in the treatment of COVID-19,which provided a new perspective for the study of the mechanism of traditional Chinese medicine(TCM)in the treatment of COVID-19.