作者认为巨噬细胞之所以能表现出细胞杀伤或细胞抑制效应是与靶细胞未确定的特性相关。为此作者研究了短小棒状杆菌(CP)激活的或正常的小鼠腹腔巨噬细胞对处于细胞周期不同时相的EMT-6小鼠腺肉瘤细胞的细胞抑制效应。激活的巨噬细胞对该瘤株只有细胞抑制效应且作用是可逆的;作者用有丝分裂选择法获得大量同步化的EMT-6肿瘤细胞,由~3H-TdR掺入及细胞计数确定该瘤株细胞周期的不同时相。在靶细胞不同时相加入0.5毫升效应细胞(4×10~(?)/毫升),1小时后除去不粘附的细胞,加入新鲜培养液。在一 The authors believe that macrophages can exhibit cell killing or cytostatic effects that correlate with the undefined characteristics of target cells. The authors studied the cytostatic effects of C. parvum (CP)-activated or normal mouse peritoneal macrophages on EMT-6 mouse adenoma cells at different cell cycle phases. Activated macrophages had only cytostatic effect on this tumor strain and the effect was reversible; the authors used mitotic selection to obtain a large number of synchronized EMT-6 tumor cells, which were identified by ~3H-TdR incorporation and cell counts. The different phases of the cycle. 0.5 ml of effector cells (4×10 −4 /ml) were added to the target cells at different times. Non-adherent cells were removed after 1 hour and fresh medium was added. In a