论文部分内容阅读
采用Lys-Sepherose 4B亲和层析 ,从新生牛血清中分离提纯了牛纤溶酶原 ( plasminogen ,pgn) .利用SDS -聚丙烯酰胺凝胶电泳分析 ,对其纯度和相对分子质量进行了鉴定 .利用猪胰弹性蛋白酶酶切纤溶酶原获得了环状结构域K1 -3,采用Lys-Sepherose 4B亲和层析对该片段进行了分离提取
Bovine plasminogen (pgn) was isolated and purified from neonatal bovine serum by Lys-Sepherose 4B affinity chromatography, and its purity and relative molecular mass were identified by SDS-polyacrylamide gel electrophoresis . The circular domain K1 -3 was obtained by enzymatic digestion with porcine pancreatic elastase and the fragment was isolated by Lys-Sepherose 4B affinity chromatography