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目的 对 60例血清学阴性 (非甲～非庚型 )的病毒性肝炎患者进行临床特点及肝组织免疫组化分析。方法 用Menghini法穿刺取肝组织 ,10 %福尔马林固定标本 ,石蜡包埋 ,切片。采用生物素标记的第二抗体及链霉菌抗生物素连接的过氧化物酶及底物色素 (S -P法 )测定肝组织HBsAg、HBcAg、HCVAg。 结果 HBVAg阳性 3 0例 ,HCVAg阳性 5例 ,HBV、HCV重叠感染 6例 ,全阴性 19例。结论 HBV、HCV为血清学全部阴性肝炎的主要病原 ,对血清学检查病原阴性病毒性肝炎应重视肝组织病理学及病原学的检查。
Objective To investigate the clinical features and immunohistochemical analysis of liver tissue in 60 seronegative (non-A to non-G) viral hepatitis patients. Methods The liver tissue was punctured with Menghini method and fixed in 10% formalin, embedded in paraffin and sliced. HBsAg, HBcAg and HCVAg in liver tissue were determined by biotinylated secondary antibody and Streptomyces avidin linked peroxidase and substrate pigment (S-P method). Results HBVAg positive 30 cases, HCVAg positive in 5 cases, HBV, HCV overlap infection in 6 cases, all negative in 19 cases. Conclusions HBV and HCV are the major pathogens of all seronegative hepatitis. Serological examination of pathogenic negative viral hepatitis should pay attention to the examination of liver histopathology and etiology.