核磁共振法常规应用于解析化学物质结构和反应性能 ,现已发展成为生物化学和分子生物学的重要实验技术 ,尤其是在确定溶液中蛋白和多肽结构方面起着其它物理分析方法不可比拟的作用。文章综述了化学位移 ,偶合常数 ,核 Overhauser效应 ( NOE)以及同位素交换等确定蛋白或多肽二级结构的方法。 The routine application of NMR in the analysis of chemical structure and reactivity has become an important experimental technique in biochemistry and molecular biology. It plays an incomparable role in determining the structure of proteins and peptides in solution in particular. . In this paper, the methods for determining the secondary structure of a protein or polypeptide, such as chemical shifts, coupling constants, nuclear Overhauser effect (NOE) and isotope exchange, are reviewed.