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目的:利用肝部分切除术,建立可准确量化和易于操控的小鼠肝脏再生模型,为研究肝脏再生的细胞和分子生物学机制及其病理学意义提供技术平台。方法:正常成年C57BL/6小鼠经心脏灌注固定后,逐叶分离肝脏、称重,计算各肝叶所占肝重的百分比;麻醉状态下,分叶顺次无菌切除小鼠肝脏的左外叶、左中叶和右中叶,建立肝脏大部切除的再生模型;利用RT-PCR方法动态检测术后肝脏甲胎蛋白基因的激活表达特征。结果:肝左外叶、左中叶和右中叶合计约占小鼠肝脏总重量的68%,分叶顺次切除上述3个肝叶,术后动物恢复和存活状态良好,RT-PCR结果证实甲胎蛋白基因在小鼠肝脏再生中的激活表达。结论:利用分叶顺次肝切除术,成功建立了小鼠的肝大部切除后再生模型,该方法可准确量化肝脏切除的程度,具有可控性强、简便易行和成功率高等优点,为小鼠肝再生的研究奠定了基础。
OBJECTIVE: To establish a mouse liver regeneration model that can be accurately quantified and easily manipulated by partial hepatectomy, and provide a technological platform for studying the cellular and molecular biological mechanism of liver regeneration and its pathological significance. Methods: Normal adult C57BL / 6 mice were perfused with cardioplegia, the livers were separated by leaves and weighted, and the percentage of liver weight occupied by each lobe was calculated. Under anesthesia, the leaves of the mice were aseptically removed The middle lobe, middle lobe, left middle lobe and right middle lobe. The model of liver regeneration was established by RT-PCR. The activation expression of hepatic alpha-fetoprotein gene was detected by RT-PCR. Results: The left lateral lobe, left middle lobe and right middle lobe accounted for about 68% of the total liver weight in mice. The lobes were resected in three lobes in succession. The animals recovered and survived well after operation. The results of RT-PCR confirmed that A Activation of Fetoprotein Gene in Mouse Liver Regeneration. CONCLUSION: Subcutaneously posterior hepatic resection model of mice is established successfully with lobulated sequential hepatectomy. This method can accurately quantify the degree of hepatic resection and has the advantages of strong controllability, simple and easy operation and high success rate. The study of mouse liver regeneration laid the foundation.