Study on the preparation and biodistribution of ~(99m)Tc-HMIBP

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99mTc-HMIBP, a new bone-imaging agent, was prepared by the reduction of 99mTc-pertechnetate in the presence of SnCl2?2H2O. The effects of the amounts of SnCl2?2H2O and HMIBP and the pH value on the labeling yield and radiochemical purity of 99mTc-HMIBP were investigated. When the amounts of SnCl2?2H2O and HMIBP were more than 10 μg and 2.5 mg, respectively, the pH value was between 2 and 7, and the labeling reaction contin- ued for 10 min, both labeling yield and radiochemical purity of 99mTc-HMIBP were more than 90%. The biodistribu- tion in rats and bone scan in rabbits were also studied. The results showed that the bone uptake is up to 7.94%ID/g at 30 min after injection of 99mTc-HMIBP, bone-to-muscle and bone-to-blood uptake ratios were 20.89 and 16.89, re- spectively. The clear bone image was obtained at 120 min after injection of 99mTc-HMIBP and clearance in soft tissue was visible. All of the above-mentioned results suggested that 99mTc-HMIBP may be a potential bone-imaging agent. 99mTc-HMIBP, a new bone-imaging agent, was prepared by the reduction of 99mTc-pertechnetate in the presence of SnCl2 · 2H2O. The effects of the amounts of SnCl2 · 2H2O and HMIBP and the pH value on the labeling yield and radiochemical purity When the amounts of SnCl2 · 2H2O and HMIBP were more than 10 μg and 2.5 mg, respectively, the pH value was between 2 and 7, and the labeling reaction was repeated for 10 min, both labeling yield and radiochemical purity of 99mTc-HMIBP were more than 90%. The biodistribu- tion in rats and bone scan in rabbits were also studied. The results showed that the bone uptake is up to 7.94% ID / g at 30 min after injection of 99mTc -HMIBP, bone-to-muscle and bone-to-blood uptake ratios were 20.89 and 16.89, re- spectively. The clear bone image was obtained at 120 min after injection of 99mTc-HMIBP and clearance in soft tissue was visible. All of the above-mentioned results suggested that 99mTc-HMIBP may be a potential bone-imaging age nt.
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