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本文用电子显微镜观察了树鼩高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的形态和颗粒大小;用分光光度计和园二色仪研究了HDL和LDL的光谱特性和园二色性;用四甲基脲电泳、SDS-凝胶电泳和聚焦电泳分离了HDL中载脂蛋白的种类和含量。其结果如下: 一、电子显微镜观察HDL、LDL的形态和颗粒大小:结果证明,树鼩血清HDL和HDL均呈园球形,大小比较均匀。HDL的平均直径约为98A,80％以上的直径在80A—110A之间。统计结果表明,LDL的颗粒直径为196A,其中直径在160A—220A的颗粒约占84％。这表明LDL比较均一。 二、树鼩血清HDL的分析超速离心行为:结果表明,树鼩血清LDL和HDL在分析超速离心分析时,只呈现一个单一的
In this paper, the morphology and particle size of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in tree shrews were observed by electron microscopy. The spectral characteristics of HDL and LDL The type and content of apolipoprotein in HDL were separated by tetramethylurea electrophoresis, SDS-gel electrophoresis and focused electrophoresis. The results are as follows: First, the electron microscope observation of HDL, LDL morphology and particle size: The results show that tree shrews serum HDL and HDL showed spherical spherical shape, the size is more uniform. The average diameter of HDL is about 98A, and the diameter of more than 80% is between 80A-110A. The statistical results show that the particle diameter of LDL is 196A, of which about 84% of the particles have a diameter of 160A-220A. This shows that LDL is more homogeneous. Second, tree shrew serum HDL analysis of ultracentrifugation behavior: The results show that tree shrew serum LDL and HDL in the analysis of ultracentrifugation analysis, showing only a single