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选用‘椪柑’、‘锦橙’、‘桃叶橙’、‘华农本地早’花后7周幼果胚珠进行培养.其胚状体发生的百分率分别是:48.5%、21.2%、19.8%、6.4%;胚性愈伤组织则为:28.2%、13.5%、15.8%、5.8%.各品种胚状体和胚性愈伤组织发生的百分率明显不同.与品种胚数多少相一致.不同激素和有机物对柑桔胚珠培养、胚状体的发生及胚性愈伤组织形成产生不同的影响.多单胚的‘华农本地早’仅从基本培养基中产生胚状体和胚性愈伤组织.胚珠培养中,胚状体发生的方式,根据来源不同分为三种:第一种是由胚珠(或已黄的球形胚上)长出白色的胚性愈伤组织,继而产生胚状体:第二种是在已分化的幼苗下胚轴或根部产生胚性愈伤组织和胚状体;第三种是在畸形胚上直接不断的分化出胚状体.
The embryos were cultured for 7 weeks after young fruits such as ’Ponkanne’, ’Jincheng’, ’Peach Blossom Orange’ and ’Huahong Local Early’ flower.The percentage of embryoid body was 48.5%, 21.2%, 19.8%, 6.4% ; Embryogenic callus were: 28.2%, 13.5%, 15.8%, 5.8% .The percentage of embryoid body and embryogenic callus in each variety was significantly different, which was consistent with the number of embryos in different varieties.Hormone and organic matter Different effects on embryo culture, embryogenesis and embryogenic callus formation were observed in citrus. The embryos of ’Huahong local early’ from multiple single embryos only produced embryoid bodies and embryogenic callus from basal medium. During embryogenesis, the mode of embryogenesis is divided into three types according to different sources: the first is embryogenic callus which grows white from ovules (or yellow globular embryos), and then produces embryoid bodies: The two are in the differentiated seedlings hypocotyls or roots produce embryogenic callus and embryoid body; the third is in the embryo directly deformed embryos on the continuous differentiation.