论文部分内容阅读
目的 探讨 p16蛋白和 Ki- 6 7抗原在胃癌发生、发展中的作用。方法 应用免疫组织化学法检测 p16和 Ki- 6 7在 2 0例正常胃组织和 6 0例胃癌组织中的表达。结果 胃癌组织中 p16蛋白表达阳性率(48.9% )明显低于胃正常组织 (90 % )。 p16蛋白表达与胃癌的分化程度、淋巴结转移及浆膜和浆膜外脏器的累及有关 (P<0 .0 5 )。Ki- 6 7在胃癌组织中的标记阳性率明显高于胃正常组织 (P<0 .0 5 ) ,伴有淋巴结转移的胃癌组织Ki- 6 7标记阳性率较高。 p16蛋白表达与 Ki- 6 7分级呈明显负相关 (P<0 .0 5 ) ,p16阴性表达的细胞增生活性明显增高。结论 p16蛋白表达缺失及其引起的细胞周期调控失常在胃癌的发生、发展中起一定作用 ;p16蛋白的检测有助于估计胃癌患者的预后、指导临床治疗 ;联合检测 p16和 Ki- 6 7对判断淋巴结转移的危险性具有较高的价值
Objective To investigate the role of p16 protein and Ki-67 antigen in the development and progression of gastric cancer. Methods Immunohistochemistry was used to detect the expression of p16 and Ki-67 in 20 cases of normal gastric tissues and 60 cases of gastric cancer tissues. Results The positive rate of p16 protein expression in gastric cancer tissues (48.9%) was significantly lower than that in normal gastric tissues (90%). The expression of p16 protein was related to the degree of differentiation of gastric cancer, lymph node metastasis, and involvement of serosa and extraserosal organs (P<0.05). The positive rate of Ki-67 in gastric cancer tissues was significantly higher than that in normal gastric tissues (P<0.05), and the positive rate of Ki-67 in gastric cancer tissues with lymph node metastasis was higher. The expression of p16 protein was negatively correlated with the Ki-67 grading (P<0.05). The p16-negative cell proliferation activity was significantly increased. Conclusion The loss of p16 protein expression and the abnormal cell cycle control play a role in the occurrence and development of gastric cancer. The detection of p16 protein is helpful to estimate the prognosis of gastric cancer patients and guide clinical treatment; combined detection of p16 and Ki-67 pairs It is of high value to determine the risk of lymph node metastasis