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通过单因素分析,对不同溶剂、温度和时间条件下提取葡萄藻微量藻粉中碳氢化合物的效果进行了比较,获得了最佳提取条件为:40 mg藻粉悬浮于正己烷中,超声处理(35℃,70 Hz)半小时后再于35℃下提取10 h,碳氢化合物得率最高,占藻粉干重的2.95‰。比较获得了GC-MS分析的程序升温优化条件,发现处于对数期的葡萄藻微量冻干藻粉中含量最高的碳氢化合物都是C22-C31长链烷烃,其组成与含量分别为:二十二烷(50.40μg/g)、二十四烷(209.17μg/g)、二十五烷(351.45μg/g)、9-辛基十七烷(472.38μg/g)、二十七烷(540.48μg/g)、二十八烷(542.24μg/g)、角鲨烯(473.60μg/g)、正三十一烷(306.86μg/g)。该提取和分析过程具有样品量少、时间短、得率高和操作简单的优点,可作为葡萄藻碳氢化合物分析检测的一种快速高效途径。
By single factor analysis, the effect of extracting the hydrocarbons in the microalga algae powder of algae by different solvents, temperature and time were compared. The optimum extraction conditions were obtained as follows: 40 algae powder was suspended in n-hexane, sonicated (35 ℃, 70 Hz) for half an hour and then extracted at 35 ℃ for 10 h. The yield of hydrocarbon was the highest, accounting for 2.95 ‰ of the dry weight of algal flour. The optimal temperature-programmed conditions for GC-MS analysis were compared. It was found that the highest content of C22-C31 long-chain hydrocarbons in the logarithmic phase of the microalgal freeze-dried algae was composed of di (50.40 μg / g), tetracosane (209.17 μg / g), pentacosane (351.45 μg / g), 9-octylheptadecane (472.38 μg / g) (540.48 μg / g), octacosane (542.24 μg / g), squalene (473.60 μg / g) and n-undecane (306.86 μg / g). The extraction and analysis process has the advantages of small sample amount, short time, high yield and simple operation, and can be used as a fast and efficient method for the analysis and detection of grape algae hydrocarbons.