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本文拟定的纸色谱法是以苯-乙酸乙酯-甲醇-乙酸(9:2:1:1)作展开剂,将血卟啉衍生物(HPD)分离为五部份,分别测定各部份浸取液的荧光发射光谱,作为一种对血卟啉衍生物组份作对比鉴定的参考方法。采用Ward的高效液相色谱程序对比研究结果表明,国产与美国产HPD的组份近似,均以血卟啉为主要组份,而前者血卟啉的含量较高;二者还均含有羟乙基乙烯基衍生物和羟基乙酸酯,初步研究了血卟啉衍生物在甲醇-水溶剂系统中的高效液相色谱行为,进一步将血卟啉衍生物分离为九部份,有利于单一组份研究。
Paper prepared by this paper is benzene - ethyl acetate - methanol - acetic acid (9: 2: 1: 1) as a developing agent, the hematoporphyrin derivative (HPD) was separated into five parts, were measured in each part Fluorescence emission spectra of leaching solution as a reference method for the comparative identification of hematoporphyrin derivative components. The results of comparative studies using Ward HPLC showed that the components of HPD in China and the United States were similar and both hematoporphyrin was the main component while the former was higher in hematoporphyrin. Both of them also contained hydroxyethyl Based vinyl derivatives and glycolic acid esters, preliminary study of hematoporphyrin derivatives in methanol - water solvent system, high performance liquid chromatography, and further separation of hematoporphyrin derivatives into nine parts, is conducive to a single group Research.