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研究小檗碱对豚鼠心肌细胞胞浆 [Ca2 + ]i 的影响 .用酶解法分离单个豚鼠心肌细胞 ,Fluo- 3/AM标记胞浆内游离钙离子 ,激光扫描共聚焦显微镜实时测定胞浆 [Ca2 + ]i变化 .结果 ,小檗碱 1 -1 0 0 μmol· L-1对正常台氏液中静息状态细胞胞浆[Ca2 + ]i 无明显影响 ;小檗碱 1 μmol·L-1对 KCl30mmol·L-1引起的细胞外钙内流有抑制作用 ( P<0 .0 1 ) ,而小檗碱 1 0 0 μmol·L-1对细胞内钙库释放有强烈激动作用 ( P<0 .0 1 ) .结果提示在豚鼠心肌细胞上低浓度小檗碱即能抑制电压依赖性钙通道 ,而高浓度时又能激动细胞内钙库释放 .
To study the effect of berberine on the cytoplasmic [Ca2 + ]i of guinea pig cardiomyocytes. Single guinea pig cardiomyocytes were isolated by enzymolysis, free intracellular calcium ions were labeled by Fluo-3/AM, and the cytoplasm was measured by laser scanning confocal microscopy. Changes in Ca2 + ]i. Results Berberine 1 -1 0 0 μmol·L-1 had no effect on cytosolic [Ca2 + ]i in normal Tyrode’s solution; Berberine 1 μmol·L- 1There was an inhibitory effect on extracellular calcium influx induced by KCl 30mmol·L-1 (P<0.01), while berberine 100 μmol·L-1 had a strong agonistic effect on intracellular calcium release (P <0·0 1 ). The results suggest that low concentrations of berberine on guinea pig cardiomyocytes inhibited voltage-dependent calcium channels, but at high concentrations they also stimulated the release of intracellular calcium stores.