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目的探讨妇平胶囊对小鼠细菌性慢性盆腔炎的治疗效果及初步作用机制。方法将小鼠随机分为6组,即正常对照组、模型对照组、妇科千金胶囊组(0.48 g/kg)和妇平胶囊低、中、高(0.27 g/kg、0.54 g/kg、1.08 g/kg)剂量组,每组10只。除正常组外,其他各实验组将大肠杆菌、金黄色葡萄球菌和乙型溶血性链球菌的混合菌液经小鼠阴道—宫颈口注入小鼠子宫,复制小鼠慢性盆腔炎模型。造模7 d后,连续灌胃给药14 d。于第15天,小鼠摘眼球取血,采用ELISA法测定血清中肿瘤坏死因子(TNF-α)和白介素6(IL-6)含量;剖取小鼠脾脏和子宫,测定脾脏和子宫系数,检查子宫的病理组织学改变。结果与模型对照组比较,妇科千金胶囊组和妇平胶囊低、中、高剂量组小鼠血清TNF-α和IL-6含量显著降低,脾脏系数明显增大,子宫系数明显减小。光镜下观察显示,模型组小鼠子宫病理炎症程度明显,妇科千金胶囊组和妇平胶囊组均能抑制子宫炎症细胞,减轻子宫肿胀、充血现象。结论妇平胶囊对慢性盆腔炎小鼠具有抗炎作用,可减轻子宫肿胀度,显著下调血清中TNF-α和IL-6的含量。
Objective To investigate the therapeutic effect of Fu Ping Capsule on bacterial chronic pelvic inflammatory disease in mice and its primary mechanism of action. Methods The mice were randomly divided into 6 groups: normal control group, model control group, gynecological Qianjin capsule group (0.48 g / kg) and Fu Ping capsule low, medium and high (0.27 g / kg, 0.54 g / g / kg) dose group, 10 rats in each group. In addition to the normal group, the other experimental groups will be E. coli, Staphylococcus aureus and mixed hemolytic streptococci B cell via vaginal - cervix into the mouse uterus, replicating mouse chronic pelvic inflammatory disease model. After 7 days of modeling, continuous intragastric administration of 14 d. On the 15th day, the mice were taken out of the eyeball to take the blood and the contents of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in the serum were measured by ELISA. The spleen and uterus of the mice were taken, Check the pathological changes in the uterus. Results Compared with the model control group, the serum levels of TNF-α and IL-6 in the Gynecological Qianjin Capsule group and the Fu Ping capsule low, medium and high dose groups were significantly decreased, the spleen coefficient significantly increased, and the uterine coefficient decreased significantly. Light microscopy showed that the model group of mice with pathological inflammation of the degree of uterine obvious, gynecological Qianjin capsule group and the capsule can inhibit uterine inflammatory cells, reduce uterine swelling, congestion phenomenon. Conclusion Fu Ping Capsule has anti-inflammatory effects on mice with chronic pelvic inflammatory disease, can reduce the degree of uterine swelling, significantly reduce the serum levels of TNF-α and IL-6.