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目的:研究马黛通络饮对动脉粥样硬化(AS)小鼠模型血管内皮损伤的保护作用机制。方法:选取5周龄雄性AopE-/-鼠48只,随机分为模型组、辛伐他汀组和马黛通络饮高、低剂量组,相同遗传背景C57BL/6J雄性小鼠为正常对照组,共5组,每组12只。除正常对照组外,其余各组给予高脂饲料喂养4周造模,马黛通络饮高、低剂量组每天给予马黛通络饮煎剂20 g/(kg·d)、10 g/(kg·d)灌胃,辛伐他汀组每天给予辛伐他汀稀释液0.01 g/(kg·d)灌胃,正常对照组和模型组灌胃等体积的生理盐水。连续灌胃8周后,采用ELISA法检测各组小鼠主动脉组织中的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)、单核细胞趋化蛋白1(MCP-1)、内皮源型一氧化氮合酶(eNOS)水平,Western blot法检测核转录因子-κB(NF-κB)的水平。结果:与正常对照组比较,模型组、辛伐他汀组、马黛通络饮低剂量组、马黛通络饮高剂量组小鼠主动脉组织中的TNF-α、IL-6、ICAM-1、VCAM-1、MCP-1、NF-κB水平显著升高,eNOS显著降低,差异均有统计学意义(P<0.05,P<0.01)。与模型组比较,辛伐他汀组、马黛通络饮低剂量组、马黛通络饮高剂量组小鼠主动脉组织中的TNF-α、IL-6、ICAM-1、VCAM-1、MCP-1、NF-κB显著降低,eNOS显著升高,差异均有统计学意义(P<0.05,P<0.01)。结论:马黛通络饮通过抑制NF-κB信号通路的活性,降低Apo E基因敲除小鼠主动脉内TNF-α、IL-6、ICAM-1、VCAM-1、MCP-1等炎性因子、黏附分子、趋化因子水平,增加了eNOS的表达,达到抑制炎症浸润、保护血管内皮的作用。
Objective: To study the mechanism of maitetongluo drink on vascular endothelium injury of atherosclerosis (AS) mouse model. Methods: Forty-eight male AopE - / - mice, 5 weeks old, were randomly divided into model group, Simvastatin group and Mataitongluo high and low dose groups. C57BL / 6J male mice with the same genetic background were selected as normal control group 5 groups, 12 in each group. In addition to the normal control group, the remaining groups were given high-fat diet feeding 4 weeks modeling, Ma De Tongluo drink high and low dose group given Ma De Tong Luo decoction daily 20 g / (kg · d), 10 g / (kg · d) The rats in simvastatin group were administrated with 0.01 g / (kg · d) simvastatin solution daily, and the normal control group and model group were given the same volume of normal saline. After 8 weeks of continuous gavage, the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), intercellular adhesion molecule- 1, MCP-1 and eNOS were detected by Western blot. The expressions of nuclear factor-kappa B (NF-κB) κB (NF-κB) levels. Results: Compared with the normal control group, the levels of TNF-α, IL-6, ICAM-1, VCAM-1 in the aorta of model group, simvastatin group, 1, MCP-1, NF-κB levels were significantly increased, eNOS was significantly lower, the difference was statistically significant (P <0.05, P <0.01). Compared with model group, the levels of TNF-α, IL-6, ICAM-1, VCAM-1, MCP-1 in aortas of simvastatin group, NF-κB was significantly decreased, eNOS was significantly increased, the difference was statistically significant (P <0.05, P <0.01). Conclusion: Ma De Tong Luo drink can reduce the inflammatory factors such as TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 in the aorta of ApoE knockout mice by inhibiting the activity of NF- Adhesion molecules, chemokine levels, increased expression of eNOS, to inhibit the infiltration of inflammation, the role of protecting the vascular endothelium.