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目的筛选汞作业工人接汞早期血浆特异性microRNA(miRNA)分子。方法将研究对象分为汞吸收组和对照组,每组10人。先使用miRNA微阵列芯片分析两组miRNA表达谱,再对候选差异miRNA进行Real-Time PCR验证。结果在10个符合候选条件的microRNA中,最终选出2个miRNA:hsa-miR-16(P<0.001)、hsa-miR-451a(P<0.05),其在汞吸收组中相较于对照组显著下调,结果与芯片分析一致。结论 miR-16和miR-451a可能成为汞作业工人早期特异性分子标志物,并且可能在表观遗传水平上参与了汞在人体内的毒作用过程,但需要进一步探讨研究miRNA改变与汞暴露的因果关系。
Objective To screen mercury-exposed workers for mercury early plasma-specific microRNA (miRNA) molecules. Methods The subjects were divided into mercury absorption group and control group, each group of 10 people. Two sets of miRNA expression profiles were first analyzed using a miRNA microarray, followed by Real-Time PCR validation of candidate miRNAs. RESULTS: Of the 10 candidate microRNAs, 2 miRNAs were finally selected: hsa-miR-16 (P <0.001) and hsa-miR-451a (P < Group significantly reduced, the results consistent with the chip analysis. Conclusions miR-16 and miR-451a may be early specific molecular markers for mercury workers and may be involved in the toxicological process of mercury at the epigenetic level. However, further studies are needed to investigate the effects of miRNA changes and mercury exposure Causal relationship.