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目的：比较５种试验／探针系统检测感染兔血清中循环抗原（ＳＣＡ）的效果。方法：用单雄性和单雌性尾蚴分别感染９只家兔，以复性尾蚴感染７只家兔作对照；感染后定期采血。另１５兔分３组感染２５０条尾蚴。１，２组于感染后１９ｄ和４５ｄ分别开始给予丙烯基硫脲（２９５－５９０ｍｇ／ｋｇ）以抑制虫卵形成，第３组作对照；均定期采血。检测方法计５种：１斑点－ＥＬＩＳＡ／抗成虫表膜单抗（８ＳＥ４），２斑点－ＥＬＩＳＡ／抗ＣＣＡ单抗（３Ｄ１０），３夹心斑点－ＥＬＩＳＡ／抗虫卵单抗（ＭＧ２），４夹心法－ＥＬＩＳＡ／抗虫卵单抗（２Ｈ１０），５夹心法－ＥＬＩＳＡ／抗ＣＡＡ单抗（ＩＢ１０）。结果：９只单雄性尾蚴和９只单雌性尾蚴感染兔血清１法均为阴性，２法仅１只检出雄虫１３３条的家兔出现阳性反应，５法单雌性感染均阴性、单雄性感染３／９兔阳性。服药抑制虫卵形成的兔血清，１９ｄ开始服药５兔１法及３法均未检出ＳＣＡ，４６ｄ开始服药组在感染后６－７ｗｋ二法均检出ＳＣＡ。４法服药与未服药兔均为阴性，５法感染后６ｗｋ的血清服药与未服药兔均呈阳性反应。结论：不同的试验／探针系统的检测效果不同，但所试３种方法（１，２，５种）都不能检出单雌性感染。抑制虫卵形成?
OBJECTIVE: To compare the effects of five test / probe systems on the detection of circulating antigens (SCA) in infected rabbits. Methods: Nine rabbits were infected with single male and female respectively, and seven rabbits infected with cercariae were used as controls. Blood samples were taken periodically after infection. Another 15 rabbits divided into 3 groups infected 250 cercariae. Group 1 and group 2 received propanethiourea (295-590 mg / kg) at 19d and 45d post-infection, respectively, to inhibit egg formation. Group 3 served as control; blood was collected routinely. Five detection methods were as follows: 1 spot-ELISA / anti-adult membrane monoclonal antibody (8SE4), 2 spot-ELISA / anti-CCA monoclonal antibody (3D10), 3 sandwich spot-ELISA / Sandwich method - ELISA / pestobolomycin (2H10), 5 Sandwich method - ELISA / anti-CAA monoclonal antibody (IB10). Results: Nine rabbits with single cercariae and nine single-cercaria infected rabbits were negative in serum 1 test. In the 2 rabbits, only one of 133 rabbits tested positive for the disease. Five single-female infectives were all negative and single-male Infection 3/9 rabbit positive. Take the medicine to inhibit the formation of rabbit serum from the eggs and take the medicine on the 19th day. 5 The SCA was not detected in the rabbits 1 and 3 methods. SCA was detected in the 6-7 weeks after the infection in the 46th day. 4 law medication and non-medication rabbits were negative, 5 law infection 6wk serum medication and no medication rabbits were positive. CONCLUSIONS: Different test / probe systems have different detection effects, but none of the three tested methods (1, 2, 5) detect single female infections. Inhibition of egg formation?