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生物农药的使用极大地减少了对环境的污染,探究生物农药对致病菌的细菌多样性及群落分布将为后续研究生物农药对致病菌的微生态调控提供理论依据.本研究运用Illumina Mi Seq高通量测序技术分析比较辣椒健康植株与疫病发病植株根际土壤微生物多样性,以及研究沼泽红假单胞菌PSB06对植株根际土壤的微生物多样性影响,探究沼泽红假单胞菌PSB06对辣椒疫病的微生态调控机制.结果显示,在第7 d和第14 d的来自同一处理的根际土壤细菌群落多样性变化没有显著差异,辣椒疫病发病植株根际土壤微生物多样性均小于健康植株根际土壤微生物多样性且喷洒沼泽红假单胞菌PSB06发酵液的土样微生物多样性最高;辣椒疫病发病植株根际土壤中放线菌丰度均小于健康植株且喷洒沼泽红假单胞菌菌剂PSB06的土壤中放线菌的丰度最高.辣椒发病植株与健康植株根际土壤中微生物多样性存在显著差异.施用沼泽红假单胞菌可以改善土壤微生物区系,提高土壤微生物群落丰富性以及土壤中放线菌所占的丰度.
The use of bio-pesticides has greatly reduced the environmental pollution, to explore the bacterial diversity and community distribution of biological pesticides on pathogens will provide a theoretical basis for the follow-up study of bio-pesticides on micro-ecological control of pathogenic bacteria.In this study, Illumina Mi Seq High-throughput Sequencing Analysis of Microbial Diversity in the Rhizosphere Soil of Pepper with Healthy and Epidemic Diseases, and the Effects of Rhodopseudomonas palustris PSB06 on Rhizosphere Soil Microbial Diversity. The results showed that there was no significant difference in the bacterial community diversity in the rhizosphere soil from the same treatment on the 7th and the 14th day.The microbial diversity in rhizosphere soil of pepper blight disease was less than that of healthy The rhizosphere soil microbial diversity and the spraying Rhodopseudomonas palustris PSB06 fermentation broth had the highest diversity of soil microorganisms. The abundance of actinomycetes in rhizosphere soil of pepper blight disease-causing plants was less than that of healthy plants and spraying of Rhodopseudomonas palustris Actinomyces PSB06 had the highest abundance of actinomycetes in the soil.The microorganisms in rhizosphere soil of pepper plants and healthy plants Like the existence of significant differences administration marsh palustris can improve soil microflora, improve the soil microbial community richness and abundance of soil actinomycetes share.