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Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.
DNA fiber nanoelectrode (tip diameter ca. 100 nm) -patch clamp was initially applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma (PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.