Background: The aim of the study was to compare different methods for pupillometry, namely the Goldmann perimeter (gp), the Colvard pupillometer (cp) and the Procyon Video pupillometer (pvp). For the pvp three different illuminations were available: mesopic high, mesopic low, and scotopic. Patients: The size of the pupil was measured in 100 eyes (50 healthy subjects) with the three different methods. We examined 29 females (58% ) and 21 males (42% ) with an average age of 25.16 years, ranging from 18 to 30 years. Results: For the Goldmann perimeter, a mean pupil diameter of 4.39 mm ± 0.62 mm was found under mesopic conditions (1.40 lux). For the Colvard pupillometer for scotopic conditions (0 lux), a mean pupil diameter of 6.80 mm ± 0.81 mm was found. For pvp the pupil diameter ranged from 7.06mm ± 0.71 mm for scotopic (0.04 lux), over 6.24mm ± 0.80 mm for mesopic low (0.40 lux) to 4.65 mm ± 0.73 mm for mesopic high conditions (4.00 lux). Conclusion: The comparison of the results showed a high correlation between the Goldmann perimeter and the Procyon Video Pupillometer for mesopic high with a minimum difference of 0.25 ± 0.69 mm. By addition of 2.67 mm to the mesopic measurement of the Goldmann perimeter, the results for the Procyon Video pupillometer at the scotopic level, by addition of 2.4 mm the scotopic measurement of the Colvard pupillometer could be achieved. Background: The aim of the study was to compare different methods for pupillometry, namely the Goldmann perimeter (gp), the Colvard pupillometer (cp) and the Procyon Video pupillometer (pvp). For the pvp three different illuminations were available: mesopic high, Patients: The size of the pupil was measured in 100 eyes (50 healthy subjects) with the three different methods. We examined 29 females (58%) and 21 males (42%) with an average age of 25.16 years, ranging from 18 to 30 years. Results: For the Goldmann perimeter, a mean pupil diameter of 4.39 mm ± 0.62 mm was found under mesopic conditions (1.40 lux). For the Colvard pupillometer for scotopic conditions (0 lux), a mean The pupil diameter of 6.80 mm ± 0.81 mm was found. For pvp the pupil diameter ranged from 7.06 mm ± 0.71 mm for scotopic (0.04 lux), over 6.24 mm ± 0.80 mm for mesopic low (0.40 lux) to 4.65 mm ± 0.73 mm for mesopic high conditions (4.00 lux). Conclusion: The comparison of the results showed a high correlation between the Goldmann perimeter and the Procyon Video Pupillometer for mesopic high with a minimum difference of 0.25 ± 0.69 mm. By addition of 2.67 mm to the mesopic measurement of the Goldmann perimeter, the results for the Procyon Video pupillometer at the scotopic level, by addition of 2.4 mm the scotopic measurement of the Colvard pupillometer could be achieved.