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〔目的〕利用 2 .5Gy 90 %的中子射线照射BALB/C小鼠 ,研究小鼠血清中反映心肌细胞损伤程度的天门冬氨酸氨基转移酶 (AST)、乳酸脱氢酶 (LDH)和肌酸激酶 (CK)、丙氨酸氨基转移酶 (ALT)活力的变化情况 ,以确定 2 .5Gy中子辐射对小鼠心肌细胞损伤的情况。〔方法〕采用二级BALB/C雄性小鼠作为实验动物 ,共计 78只。实验期间在军事医学科学院实验动物中心统一饲养。将上述小鼠分为 2组 ,包括 1个对照组和 1个实验组。采用北京清华大学的中子源分别对各动物组进行 90 %中子照射 ,照射剂量为 2 .5Gy(66只 ) ,对照组 12只。分别在照射动物之后的 6h、12h、1d、3d、5d、7d、10d、14d、2 1d、2 8d和 41d采取静脉血 ,进行AST、LDH、CK和ALT的活性测定。采用MicrocalOrigin软件包进行数理统计分析。〔结果〕2 .5Gy中子照射 5d后血清AST、LDH、CK和ALT活力均有明显升高 ,直到于照射后 2 1d～ 2 8d后仍然未恢复至正常状态 ,均显著高于正常对照组 (P <0 .0 5 )。〔结论〕2 .5Gy中子辐射可以引起心肌细胞损伤 ,进而引起血清AST、LDH、CK和ALT活力不同程度的升高。
[Objective] To study the effects of 90% neutrons of 2.5Gy in BALB / C mice on the serum aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and Creatine kinase (CK), alanine aminotransferase (ALT) activity changes in order to determine the 2.5Gy neutron radiation on myocardial cell injury in mice. [Method] Two BALB / C male mice were used as experimental animals for a total of 78 animals. During the experiment in the Academy of Military Medical Experimental Animal Center reunification. The above mice were divided into 2 groups, including 1 control group and 1 experimental group. The neutron sources of Tsinghua University in Beijing were used to irradiate 90% neutron of each animal group with a dose of 2.5 Gy (66) and 12 control animals. Venous blood samples were taken at 6h, 12h, 1d, 3d, 5d, 7d, 10d, 14d, 21d, 28d and 41d after irradiation respectively to determine the activities of AST, LDH, CK and ALT. MicrocalOrigin software package for mathematical statistics. 〔Results〕 The serum AST, LDH, CK and ALT activities of serum of 5. 5 Gy neutrons were significantly increased after 5 days of irradiation, and they did not return to the normal state after 21d ~ 28d after irradiation, which were significantly higher than those of normal control group (P <0. 05). 〔Conclusion〕 2.5Gy neutron radiation can cause myocardial cell injury, which in turn lead to varying degrees of serum AST, LDH, CK and ALT increased.