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目的:建立何首乌快速繁殖体系并对之进行优化;探索组织培养条件下诱导何首乌同源四倍体的有效方法。方法:采用正交设计的方法,对何首乌的繁殖培养基进行优化筛选;将顶芽于不同浓度秋水仙碱溶液中浸泡不同时间筛选何首乌同源四倍体的诱导条件。结果:何首乌最佳繁殖培养基为MS+6-BA 1.0 mg.L-1+NAA 0.3 mg.L-1+PP3330.4 mg.L-1;0.2%秋水仙碱浸泡芽30 h,或0.3%秋水仙碱浸泡芽18 h为诱导何首乌同源四倍体的理想条件,诱导率高达16.7%。结论:采用组织培养方法可进行何首乌的快速繁殖;秋水仙碱是有效的多倍体诱导剂。通过根尖细胞染色体鉴定,共获得25个同源四倍体株系。该实验为何首乌野生资源保护、优良品种的选育奠定了基础。
OBJECTIVE: To establish and optimize the rapid multiplication system of Polygonum multiflorum. To explore the effective method of inducing autotetraploid of Polygonum multiflorum under tissue culture conditions. Methods: Orthogonal design method was used to optimize the culture medium of Radix Polygoni multiflori. The conditions for inducing the autotetraploid of Polygonum multiflorum were screened by immersing the buds in different concentrations of colchicine at different times. Results: The optimal culture medium of Polygonum multiflorum was MS + 6-BA 1.0 mg.L-1 + NAA 0.3 mg.L-1 + PP3330.4 mg.L-1; 0.2% colchicine soaked bud for 30 h or 0.3 % Colchicum soaking bud 18 h is the ideal conditions for inducing autotetraploid polygonum multiflorum, the induction rate is as high as 16.7%. Conclusion: Tissue culture method can be used for rapid multiplication of Polygonum multiflorum. Colchicine is an effective polyploid inducer. By root tip cell chromosome identification, a total of 25 autotetraploid lines were obtained. This experiment laid the foundation for the conservation of Radix Polygoni multiflori and the breeding of fine varieties.