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目的研究维生素E琥珀酸脂(Vitamin E succinate,VES)对顺铂(cis-diamminedichloroplatinum,CDDP)所致人胚肾293(HEK293)细胞毒性的保护作用。方法体外培养人胚肾293细胞(HEK293),设空白对照组、VES(20μg/ml)对照组、CDDP(25μg/ml)单独染毒组以及低剂量CDDP(25μg/ml)+VES(5μg/ml)联合处理组、中剂量CDDP(25μg/ml)+VES(10μg/ml)联合处理组、高剂量CDDP(25μg/ml)+VES(20μg/ml)联合处理组,染毒12 h。采用MTT法观察细胞增殖效应,测定细胞还原型谷胱甘肽(GSH)、丙二醛(MDA)含量和细胞培养液中乳酸脱氢酶(LDH)活力,采用荧光法检测DNA链间的交联情况。结果与CDDP单独染毒组比较,各剂量CDDP+VES联合处理组HEK293细胞的存活率均升高,差异有统计学意义(P<0.05,P<0.01)。VES能明显抑制CDDP所致HEK293细胞MDA含量和细胞培养液中LDH活力的升高,并能提高细胞GSH的含量(P<0.05,P<0.01)。高剂量CDDP+VES联合处理组HEK293细胞的交联率低于CDDP单独染毒组,差异有统计学意义(P<0.05)。结论 VES能抑制CDDP所致HEK293细胞的细胞毒性,其机制可能与VES的抗氧化作用和对DNA损伤的防护作用有关。
Objective To study the protective effect of Vitamin E succinate (VES) on cytotoxicity of human embryo kidney 293 (HEK293) induced by cisplatin (CDDP). Methods Human embryonic kidney 293 cells (HEK293) were cultured in vitro. VES (20μg / ml), CDDP (25μg / ml) alone and low dose CDDP (25μg / ml) (25μg / ml) + VES (10μg / ml) combined with high dose of CDDP (25μg / ml) + VES (20μg / ml) for 12 hours. MTT assay was used to observe the effect of cell proliferation. The contents of glutathione (GSH), malondialdehyde (MDA) and lactate dehydrogenase (LDH) activity in cell culture medium were determined. United situation. Results Compared with CDDP alone, the survival rates of HEK293 cells in all dosages of CDDP + VES combined treatment group were significantly increased (P <0.05, P <0.01). VES significantly inhibited the MDA content of HEK293 cells induced by CDDP and LDH activity in cell culture medium and increased the content of GSH (P <0.05, P <0.01). The cross-linking rate of HEK293 cells in high-dose CDDP + VES combined treatment group was lower than that of CDDP alone group, the difference was statistically significant (P <0.05). Conclusion VES can inhibit the cytotoxicity of CDDP-induced HEK293 cells. The mechanism may be related to the anti-oxidative effect of VES and the protective effect on DNA damage.