论文部分内容阅读
目的:评价两种不同来源的间充质干细胞,即骨髓干细胞(BMSC)和脂肪干细胞(ADSC)增殖和分化能力,为其将来更好的应用于细胞治疗和组织工程提供一定的理论支持和依据。方法:分别取同一只兔的髂骨骨髓和其腹股沟脂肪作为BMSC和ADSC的来源,分离培养后比较其生物学特性包括细胞的增殖能力(MTT),成骨成脂分化潜能,细胞表面抗原标记物。结果:BMSC类似纤维细胞样,呈长梭形紧密排列;而ADSC细胞整体呈旋涡状排列;但ADSC倍增时间更短,增殖速度更快;两者都具有向成骨成脂分化的潜能,但BMSC的成骨能力更好。结论:ADSC有更短地倍增时间和更快地增殖速度;两者都具有向成骨成脂分化的潜能,但相较于ADSC更强的成脂分化能力,BMSC的成骨分化能力更好。两者都可以作为组织工程的种子细胞。
OBJECTIVE: To evaluate the proliferation and differentiation of mesenchymal stem cells (BMSCs) and adipose-derived stem cells (ADSCs) from two different sources and to provide some theoretical support and basis for their further application in cell therapy and tissue engineering . Methods: The iliac bone marrow and the inguinal fat of the same rabbit were taken as the source of BMSCs and ADSCs respectively. The biological characteristics including cell proliferation ability, osteogenic differentiation potential, Things. Results: BMSCs were fibroblast-like with long fusiform arrangement, while ADSC cells were arranged in a swirling manner. However, ADSC doubling time was shorter and the proliferation rate was faster. Both of them had the potential to differentiate into osteoblasts, BMSC osteogenic ability is better. CONCLUSIONS: ADSCs have a shorter doubling time and a faster rate of proliferation; both have the potential to differentiate into osteogenic adipocytes, but BMSCs are more osteogenic and differentiated than ADSCs for greater adipogenic differentiation . Both can serve as seed cells for tissue engineering.