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采用自组装原位生长制备基于聚苯胺/CNTs纳米复合物修饰叉指电极的葡萄糖生物传感器.首先采用自组装的方式制备CNTs修饰叉指电极,并采用对甲苯磺酸掺杂聚苯胺,原位在修饰电极表面生长聚苯胺,制备了PANI/CNTs修饰电极;然后将葡萄糖氧化酶固定在修饰电极表面制备了葡萄糖生物传感器.采用拉曼光谱、扫描电子显微镜、原子力显微镜等对所制备的PANI/CNTs复合物进行了表征分析;采用循环伏安法和电化学阻抗法研究了修饰电极的电化学行为.传感器性能评价结果表明:在最优化条件下,葡萄糖浓度与响应电流在0.5~30 mmol/L范围内呈现良好的线性关系,响应灵敏度为62.17μA/(mmol·L-1),线性相关系数为0.997,检出限为0.15 mmol/L(S/N=3),并具有良好的重现性和稳定性.
The self-assembled glucose biosensor based on polyaniline / CNTs nanocomposites was prepared by self-assembled in-situ growth.Firstly, CNTs modified interdigital electrodes were prepared by self-assembly, and polyaniline doped with p-toluenesulfonic acid PANI / CNTs modified electrode was prepared on the surface of the modified electrode, then glucose oxidase was immobilized on the surface of the modified electrode to prepare glucose biosensor.The Raman spectra, scanning electron microscopy and atomic force microscopy were used to analyze the PANI / CNTs composite was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.The electrochemical performance of the modified electrode was evaluated.The results of sensor performance evaluation showed that under the optimal conditions, the glucose concentration and response current were in the range of 0.5-30 mmol / L with a linear relationship of 62.17 μA / mmol·L-1, a linear correlation coefficient of 0.997, a limit of detection of 0.15 mmol / L (S / N = 3) and a good linear relationship Reality and stability.