论文部分内容阅读
目的:研究柴苓汤对外周血单个核细胞(PBMCs)Th1/Th2特异性转录因子T-bet/GATA-3 mRNA转录的影响,从基因水平探讨其治疗自身免疫性复发性流产的机理。方法:体外分离提取外周血单核细胞,在含有不同浓度柴苓汤的培养基中培养24 h,采用实时定量PCR技术检测T-bet和GATA-3 mRNA的表达。结果:柴苓汤浓度为10 g/ml时,PBMCs的T-bet mRNA表达水平(2.89±0.84)较对照组(1.66±0.14)增高,差异有显著性(P<0.05)。用1、10、100 g/ml浓度柴苓汤分别处理,GATA-3 mRNA的表达水平与对照组比较均无明显差异。结论:柴苓汤可上调转录因子T-bet mRNA的表达,进而可能通过增强Th1细胞因子产生、纠正自身免疫性复发性流产中Th2反应异常增强的Th1/Th2失衡状态,对其发挥治疗效应。
Objective: To investigate the effect of Chai Ling Tang on the transcription of Th1 / Th2 specific transcription factor T-bet / GATA-3 mRNA in peripheral blood mononuclear cells (PBMCs) and to explore the mechanism of its treatment on autoimmune recurrent spontaneous abortion. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated and cultured in vitro. The cells were cultured in different concentrations of Chailing Decoction for 24 hours. The mRNA expression of T-bet and GATA-3 were detected by real-time PCR. Results: The T-bet mRNA expression in PBMCs was significantly higher than that in the control group (2.89 ± 0.84) at Chalinging concentration of 10 g / ml (1.66 ± 0.14, P <0.05). When treated with Chai Ling Decoction at concentration of 1, 10 and 100 g / ml, the expression of GATA-3 mRNA showed no significant difference compared with the control group. Conclusion: Chailing decoction can up-regulate the expression of T-bet mRNA, which may be used to improve the Th1 cytokine production and to correct the abnormal Th1 / Th2 imbalance of Th2 response in autoimmune recurrent spontaneous abortion.