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Burlington,佛蒙特医院医学中心对10年期间(1969年～1978年)送验的临床标本作诊断,所分离的肠道病毒与许多种疾病有关。这些疾病有:无菌性腩膜炎、手一足一口疾病、腹泻、肋肌痛、心肌炎、咽峡炎及发热。作者获得的原始病毒标本采自在佛蒙特医院医学中心看病的病人。病人的年令范围从小于1个月到65岁以上;绝大多数的标本是从儿童和青年获得的。临床标本用棉顶涂抹器采集的咽和直肠拭子,未稀释的脑背液或疱疹液体。拭子标本在补充了0.5%牛血清蛋白的小牛浸液肉汤中洗提,并迅速接种于最初的罗猴单层细
Burlington, Vermont Hospital Medical Center diagnoses clinical specimens sent over 10 years (1969-1978) and the isolated enterovirus is associated with many diseases. These diseases are: aseptic pleurisy, hands and a foot disease, diarrhea, costal muscle pain, myocarditis, angina and fever. The original samples obtained by the authors were collected from patients at the Vermont Hospital Medical Center. The patient’s order ranged from less than 1 month to over 65 years; the vast majority of specimens were obtained from children and youth. Clinical specimens Swab and rectal swabs collected with a cotton top applicator, undiluted bradycardia or herpes fluid. Swab specimens were eluted in calf infusion broth supplemented with 0.5% bovine serum albumin and rapidly inoculated on the first rhesus monolayer