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目的:探讨血浆高迁移率族蛋白-1(HMGB-1)在脓毒症患者诊断和病情评估中的临床应用价值。方法:采用酶联免疫吸附法(ELISA)测定35例体检健康者(对照组)和35例我院急诊科和重症医学科首诊的脓毒症患者血浆HMGB-1浓度,进行对比,并将脓毒症患者血浆HMGB-1浓度与其APACHEⅡ评分进行相关性分析。结果:脓毒症患者血浆HMGB-1浓度显著高于对照组[(6.89±3.40)ng/mLvs(1.71±0.36)ng/mL,P=0.00];脓毒症患者中,感染性休克患者的血浆HMGB-1浓度显著高于非感染性休克患者[(10.40±1.50)ng/mLvs(4.25±1.41)ng/mL,P=0.00];脓毒血症患者的APACHEⅡ评分与血浆HMGB-1浓度呈显著正相关性(r=0.539,P=0.001)。结论:脓毒症患者血浆HMGB-1浓度明显升高,检测血浆HMGB-1浓度可为脓毒症的诊断及病情评估提供依据。
Objective: To investigate the clinical value of high mobility group box-1 (HMGB1) in the diagnosis and assessment of sepsis. Methods: The plasma concentrations of HMGB-1 in 35 healthy individuals (control group) and 35 patients with acute sepsis in our emergency department and the department of critical care were measured by enzyme-linked immunosorbent assay (ELISA) The correlation between plasma HMGB-1 concentration and APACHEⅡscore in patients with sepsis was analyzed. Results: The plasma levels of HMGB-1 in patients with sepsis were significantly higher than those in controls [(6.89 ± 3.40) ng / mL vs (1.71 ± 0.36) ng / mL, P = 0.00] The serum HMGB-1 concentration was significantly higher than that of non-septic shock patients [(10.40 ± 1.50) ng / mL vs (4.25 ± 1.41) ng / mL, P = 0.00]; APACHEⅡ score and plasma HMGB-1 concentration There was a significant positive correlation (r = 0.539, P = 0.001). Conclusion: The plasma HMGB-1 concentration in patients with sepsis significantly increased. Detecting plasma HMGB-1 concentration may provide the basis for the diagnosis and assessment of sepsis.