为探讨小穗苎麻素对乳腺癌MDA-MB231细胞中NF-κB活化的影响,从黄背苎麻中提取生物碱类单体小穗苎麻素,通过蛋白免疫印迹实验检测小穗苎麻素对IκBα磷酸化和降解的影响以及对p65的磷酸化和核转移的影响.结果显示:小穗苎麻素呈剂量依赖性抑制了TNF诱导的NF-κB报告基因的表达,同时抑制了乳腺癌MDA-MB231细胞中IκBα的磷酸化和降解,以及p65的磷酸化和核转移.这表明,小穗苎麻素通过抑制乳腺癌MDA-MB231细胞中IκBα的磷酸化和降解以及p65的磷酸化和核转移,进而抑制了NF-κB的活化. In order to investigate the effect of spikelet ramie on the activation of NF-κB in breast cancer MDA-MB231 cells, the alkaloid spikelet ramie was extracted from the yellow ramie, and the effect of spikelet ramie on IκBαphosphate The results showed that spikelet ramie significantly inhibited the TNF-induced NF-κB reporter gene expression and inhibited the proliferation of breast cancer MDA-MB231 cells Phosphorylation and degradation of IκBα, and phosphorylation and nuclear transfer of p65, suggesting that spikelet-ramie inhibits IκBα phosphorylation and degradation in breast cancer MDA-MB231 cells and phosphorylation and nuclear translocation of p65 NF-κB activation.