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荷正电高分子聚合物可以通过静电作用与DNA寡核苷酸序列进行结合,其与单链DNA和双链DNA结合后有不同的共振光散射(RLS)信号.基于此,设计了一种杂交DNA检测探针,实现了完全互补序列与单碱基错配序列及非互补碱基序列的区分,并建立了一种简单、快速、免标记的DNA杂交检测方法.在最佳条件下,在λ=470nm处,光散射强度达到最大,并且与目标DNA浓度在5.0~500nmol/L范围内呈线性,检出限为2nmol/L.
Charge-positive polymers can bind to DNA oligonucleotides by electrostatic interaction, which have different resonance light scattering (RLS) signals after binding to single-stranded DNA and double-stranded DNA. Based on this, Hybridization DNA detection probe to achieve the complete complementary sequence and single base mismatch sequence and non-complementary base sequence to distinguish and establish a simple, rapid, tag-free DNA hybridization detection method under the best conditions, At λ = 470 nm, the intensity of light scattering reached the maximum, and the linearity was linear with the concentration of target DNA in the range of 5.0-500 nmol / L with the detection limit of 2 nmol / L.