论文部分内容阅读
以稻瘟病 (Magnarporthe grisea)菌株 ZA49、131为供试菌株 ,进行原生质体的制备试验。结果表明 :用9种酶均能消化该菌细胞壁 ,并获得一定数量的原生质体 ;产生原生质体效率最高的是酶 Novozym TM2 34和Driselase,且这两种酶以 10 mg/ ml浓度产生的原生质体数量最多 ,消化 2～ 3 h后即可获得相当数量的原生质体 ,最适作用温度分别为 30℃和 32℃。以几丁质降解酶与 Novozym TM2 34或 Driselase混用时对制备原生质体有很好的增效作用。作者认为 ,Novozym TM2 34和 Driselase的作用浓度以 5 m g/ ml最为经济、实用。所制备的原生质体均能再生菌丝和具有产生与原菌株相同致病性分生孢子的能力。
Protoplast preparation was carried out using Magnarporthe grisea strain ZA49,131 as test strain. The results showed that all nine enzymes could digest the cell wall of bacteria and obtain a certain number of protoplasts. The protoplasts that produce the most protoplasts are the enzymes Novozym TM2 34 and Driselase, and the two enzymes produce protoplasts at a concentration of 10 mg / ml The number of the largest body, after digestion 2 ~ 3 h to obtain a considerable number of protoplasts, the optimum temperature were 30 ℃ and 32 ℃. When chitin degrading enzyme is mixed with Novozym TM2 34 or Driselase, it has good synergistic effect on protoplast preparation. The author believes that Novozym TM2 34 and Driselase the most effective concentration of 5 m g / ml, practical. The prepared protoplasts regenerated mycelium and had the ability to produce the same pathogenic conidia as the original strain.