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以巴戟天无菌苗的茎尖、带节茎、无节茎为材料进行离体培养。结果表明 :外植体表面消毒采用70 %乙醇预处理 30s ,再用 0 .1%的升汞浸泡 10～ 15min ,效果较好。茎尖和带节茎培养 ,于含有 6-苄基氨基嘌呤 ( 6-benzylaminopurine。BA)的MurashigeandTucker (MT)培养基上 ,均能直接出芽 ,带节茎出芽率最高 ,为 97.5%。适宜的BA浓度为 1mg·L- 1,BA浓度升高 ,出芽率下降。根的诱导 ,以MT加 0 .2～0 .5mg·L- 1的萘乙酸 (naphthleneaceticacid ,NAA)培养基较好 ,生根率可达 80 .0 %以上
With Morinda officinalis shoot tips, with stems, non-stems for the material culture in vitro. The results showed that the explants surface disinfection with 70% ethanol pretreatment 30s, and then 0.1% mercury chloride soak 10 ~ 15min, the better. The stem tips and stems were cultured on Murashige and Tucker (MT) medium containing 6-benzylaminopurine.BA, and all of them could shoot directly, with the highest stem budding rate of 97.5%. Appropriate concentration of BA was 1 mg · L -1, BA concentration increased, budding rate decreased. Root induction, with MT plus 0.2 ~ 0.5mg · L- 1 naphthleneacetic acid (NAA) medium is better, the rooting rate of up to 80.0% or more