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采用营养液栽培,以盐敏感型番茄品种M82为试材,利用双向电泳(2-DE)研究盐胁迫处理下幼苗叶片蛋白质的表达谱,并采用基质辅助激光解析飞行时间串联质谱(MALDI-TOF/TOF-MS)技术进行差异蛋白质的分离及质谱鉴定。结果表明:(1)盐胁迫处理下,利用2-DE获得差异显著蛋白点20个,其中17个蛋白质点丰度上调表达,3个蛋白质点丰度下调表达。(2)通过质谱分析和蛋白质NCBInr数据库检索,共鉴定出19个差异蛋白,分别为果糖-二磷酸醛缩酶、S-腺苷甲硫氨酸合成酶、甘油醛-3-磷酸脱氢酶等及3个功能未知蛋白;这些鉴定出的差异蛋白质与能量代谢、光合作用、蛋白合成、氧化还原平衡等过程相关,暗示所分离鉴定的蛋白可能参与了番茄的盐胁迫响应,为进一步研究番茄抗逆机制奠定基础。
Using nutrient solution culture, salt-sensitive tomato variety M82 was used as experimental materials. The expression profiles of seedling leaves under salt stress were studied by two-dimensional gel electrophoresis (2-DE) and analyzed by matrix-assisted laser desorption / ionization time of flight tandem mass spectrometry / TOF-MS) technology for differential protein separation and mass spectrometry identification. The results showed as follows: (1) Under salt stress treatment, 20 protein spots with significant difference were obtained by 2-DE, of which 17 protein spots were up-regulated and 3 spots were down-regulated. (2) A total of 19 differential proteins were identified by mass spectrometry and protein NCBInr database, which were fructose-diphosphate aldolase, S-adenosylmethionine synthetase, glyceraldehyde-3-phosphate dehydrogenase These proteins were identified as energy metabolism, photosynthesis, protein synthesis, redox balance and other processes, suggesting that the isolated and identified proteins may be involved in the salt stress response of tomato, for further study of tomato Anti-convalescent mechanism to lay the foundation.