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目的：研究银杏叶提取物（ＧｂＥ）对溶血磷脂酰胆碱（ＬＰＣ）引起的小牛脑微血管内皮细胞（ＢＣＭＥＣ）损伤及小牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖的保护作用。方法：以乳酸脱氢酶（ＬＤＨ）释放量为指标测定ＢＣＭＥＣ损伤，用结晶紫染色法测定ＢＣＭＳＭＣ增殖。结果：ＬＰＣ５０ｎｍｏｌ／Ｌ可明显增加ＬＤＨ的释放，ＧｂＥ０，５，１０，２０，５０和１００ｍｇ／Ｌ，则ＬＤＨ释放率由４７．０％±０．７％分别下降为３９％±３％，３６．０％±１．３％，２７．０％±２．１％，１６％±６％和１３％±３％，表明ＧｂＥ呈剂量依赖性地抑制了ＬＤＨ的释放。ＧｂＥ对ＬＰＣ引起的ＢＣＭＳＭＣ的增殖抑制作用不明显。结论：ＧｂＥ对ＬＰＣ引起的ＢＣＭＥＣ损伤具明显的保护作用
Objective: To study the protective effect of Ginkgo biloba extract (GbE) on the injury of bovine cerebral microvascular endothelial cells (BCMEC) and the proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC) induced by lysophosphatidylcholine (LPC). Methods: BCMEC injury was determined by the release of lactate dehydrogenase (LDH), and the proliferation of BCMSMC was determined by crystal violet staining. Results: LPC50nmol / L could significantly increase the release of LDH. The release rates of LDH decreased from 47.0% ± 0.7% to 39% ± 3% at GbE0, 5, 10, 20, 50 and 100 mg / .0% ± 1.3%, 27.0% ± 2.1%, 16% ± 6% and 13% ± 3%, respectively, indicating that GbE inhibits LDH release in a dose-dependent manner. GbE on LPC induced proliferation inhibition of BCMSMC is not obvious. Conclusion: GbE has a significant protective effect on LPC induced BCMEC injury