以军曹鱼头为原料,ACE抑制率为指标,对木瓜蛋白酶水解鱼头蛋白的工艺条件进行优化,筛选具有较高抑制ACE活性的生物功能活性肽。结果表明:优化水解工艺条件为温度73℃、初始pH值6.0、加酶量2300U/g,料液比1∶2,水解时间4h,水解产物对ACE抑制率达到最大值为82.63%,此时水解度为17.21%。 Using the head of cobia as raw material and the ACE inhibition rate as an index, the technological conditions for papain hydrolysis of the head protein were optimized, and biologically active peptides with high ACE inhibitory activity were screened. The results showed that the optimal hydrolysis conditions were as follows: temperature 73 ℃, initial pH value 6.0, enzyme dosage 2300U / g, solid-liquid ratio 1: 2 and hydrolysis time 4h, the maximal inhibition rate of hydrolyzate to ACE was 82.63% Degree of hydrolysis was 17.21%.