论文部分内容阅读
对鹿蹄草素作用下的金黄色葡萄球菌生长曲线、膜通透性和结构进行了研究,探讨鹿蹄草素抑菌和杀菌机理。鹿蹄草素能够有效抑制金黄色葡萄球菌的生长,其最小抑菌质量浓度为0.16mg/mL;鹿蹄草素对金黄色葡萄球菌具有较强的杀菌作用,最小杀菌质量浓度为0.2mg/mL。鹿蹄草素作用于金黄色葡萄球菌后,细胞膜通透性与空白对照组相比显著提高。扫描电镜的结果表明,鹿蹄草素作用后菌体细胞结构被破坏,细胞壁呈溶解状,菌体细胞间相互粘结,细胞与细胞间的界限变得模糊。试验结果表明,鹿蹄草素的抑菌性和杀菌功能与其对金黄色葡萄球菌细胞膜和细胞壁结构的破坏直接相关。
The growth curve, membrane permeability and structure of Staphylococcus aureus were studied under the action of genistein, and the antimicrobial and bacteriostasis mechanisms of gentiana were discussed. Salvia miltiorrhiza can effectively inhibit the growth of Staphylococcus aureus, the minimum inhibitory concentration of 0.16mg / mL; Salvia prime Staphylococcus aureus has a strong bactericidal effect, the minimum bactericidal concentration of 0.2mg / mL. Compared with the blank control group, the cell membrane permeability increased significantly after treated with Stachydrine in Staphylococcus aureus. The results of scanning electron microscopy showed that the cell structure of the cell was destroyed, the cell wall was dissolved, the cell bodies were bound to each other, and the boundary between cells and cells became blurred. The results showed that the bacteriostasis and bactericidal activity of PLD were directly related to the destruction of cell membrane and cell wall structure of Staphylococcus aureus.