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目的:通过反转录病毒转染技术成功建立了人诱导分化多能干细胞系(induced pluripotency stem cell,iPSC),并诱导它们向滋养层细胞定向分化,为研究人早期滋养层细胞的发育建立一种新的模型。方法:采用反转录病毒将八聚体结合转录因子-4(octamer-binding transcription factor-4,OCT4)、性别决定Y区域转录因子2[sex determining region Y(SRY)-box2,SOX2]、原癌基因c-Myc和Kruppel样因子4(Kruppel-like factor 4,KLF4)转染到人肺部成纤维细胞中,经过筛选得到重编程后的人iPSC,然后用骨成型蛋白4(bone morphogenetic protein 4,BMP4)将其诱导成为滋养层细胞。结果:通过该病毒转染体系成功获得iPSC,具有与人胚干细胞(human embryonic stem cell,hESC)相似的形态学及多向分化潜能,并成功将其诱导分化成为滋养层细胞。结论:通过重编程技术成功建立人iPSC细胞系,并具备与hESC相似的干细胞特性,为进一步研究胚胎早期滋养层细胞发育以及滋养层细胞恶性肿瘤的发生机制提供了一个新的平台。
OBJECTIVE: To establish a successful induced pluripotency stem cell (iPSC) by retroviral transfection and to induce them to differentiate into trophoblast cells for the purpose of establishing a human early trophoblast cell development New model. Methods: The octamer-binding transcription factor-4 (OCT4) and sex determining region Y (SRY) -box2, SOX2 were determined by retroviruses. Human lung fibroblasts were transfected with oncogene c-Myc and Kruppel-like factor 4 (KLF4), and the reprogrammed human iPSCs were screened. Then, bone morphogenetic protein 4 4, BMP4) induces them to become trophoblast cells. Results: iPSC was successfully obtained by this virus transfection system. It had similar morphological and multidirectional differentiation potential as human embryonic stem cells (hESC) and successfully induced it to differentiate into trophoblast cells. CONCLUSION: The human iPSC cell line has been successfully established by reprogramming technology and has similar characteristics of stem cells as hESC, which provides a new platform for further study of embryonic trophoblast development and trophoblastic malignancy.