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为了解三苯氧氨(Tamoxifen)和雌二醇(E_2)对原癌基因c-myc表达的影响,在体外培养的兔子宫内膜细胞(M-60),观察到给予E_(2b)小时后,原癌基因c-myc表达明显增加。E_2浓度在10~(-10)～10~(-6)mol/L范围内,c-myc的表达随E_2浓度加大而增强。Tamoxifen可拮抗E2所引起的c-myc的表达.当Tamoxifen的浓度为10~(-5)mol/L时抑制作用最明显,Tamoxifen的最佳抑制浓度是E_2浓度的10倍。本结果揭示在体外培养系统,E_2对子宫内膜细胞的增殖作用也可能是通过c-myc基因中介的。抑制c-myc基因的表达是Tamoxifen拮抗E_2刺激子宫内膜细胞增殖的原因之一。
To investigate the effect of Tamoxifen and E2 on the expression of proto-oncogene c-myc, rabbit EEC (M-60) cells cultured in vitro were observed for E 2 hours After the proto-oncogene c-myc expression increased significantly. E 2 concentration in the range of 10 ~ (-10) ~ 10 ~ (-6) mol / L, c-myc expression increased with the E 2 concentration increased. Tamoxifen can antagonize the expression of c-myc caused by E2, and the most obvious inhibition when Tamoxifen concentration is 10 -5 mol / L, Tamoxifen the best inhibitory concentration is 10 times that of E 2. The results revealed that in vitro culture system, E 2 proliferation of endometrial cells may also be mediated through the c-myc gene. Inhibition of c-myc gene expression is one of the reasons that Tamoxifen antagonizes the proliferation of endometrial cells stimulated by E 2.