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目的在保证尼氏染色、免疫组化、免疫荧光等实验效果的前提下,节省灌注时间及灌注耗液量。方法自剑突沿着胸骨中线剪至锁骨切迹,剪开皮肤,充分分离皮下组织,暴露两侧胸廓。充分暴露心脏,在距离主动脉弓1.5cm左右处用动脉夹夹闭胸主动脉。使用新型灌注针进针左心室,灌入生理盐水。剪开右心耳,将灌注板向右侧倾斜30°,当右心耳流出清亮液体时,换4%多聚甲醛,先快后慢,评估颈部及双上肢僵硬,完成固定。结果采用此改良后方法,使用新型的灌注固定针,能够在保证固定效果的前提下,显著缩短灌注固定的时长,明显减少灌注固定的耗液量。
Objective To save the perfusion time and perfusion fluid under the premise of ensuring the effect of Nissl staining, immunohistochemistry and immunofluorescence. Methods Since the xiphoid cut along the sternum midline to the clavicle notch, cut the skin, the full separation of subcutaneous tissue, exposing both sides of the thorax. Full exposure of the heart, at a distance of about 1.5cm from the aortic arch at the artery clip folder closed the thoracic aorta. Use the new perfusion needle needle into the left ventricle, filled with saline. Cut the right auricle, the perfusion plate tilted to the right 30 °, when the right atrial appendices clear liquid, 4% paraformaldehyde for the first fast and slow, neck and upper extremity assessment of stiffness, to complete the fixed. Results Using this improved method, the new perfusion fixing needle can significantly shorten the duration of perfusion fixation and significantly reduce the consumption of fixed perfusion under the premise of ensuring the fixation effect.