论文部分内容阅读
采用高效毛细管电泳对猪脑中提取的钙调素与猪胰腺中提取的磷脂酶Ａ２进行分离分析。以Ｂｉｏ－Ｒａｄ２０ｃｍ×２５μｍｃｏａｔｅｄ石英毛细管为分离柱，ＵＶ２００ｎｍ为检测波长，选择０．０５ｍｏｌ／Ｌ磷酸盐缓冲液（ｐＨ３．５）为钙调素电泳分离缓冲液体系，０．０５ｍｏｌ／Ｌ磷酸盐缓冲液（ｐＨ２．５，内含０．１％ＨＰＭＣ）为磷脂酶Ａ２电泳分离缓冲液体系，对多步分离纯化获得的钙调素与磷脂酶Ａ２进行纯度分析，同时为进一步研究钙调素与磷脂酶Ａ２的生物活性提供了有效的检测手段。
High-performance capillary electrophoresis was used to separate the calmodulin extracted from porcine brain and phospholipase A2 extracted from porcine pancreas. Using Bio-Rad 20cm × 25μmcoated quartz capillary as the separation column, UV200nm as the detection wavelength, 0.05mol / L phosphate buffer (pH3.5) was selected as calmodulin electrophoresis separation buffer system, 0.05mol / L phosphate buffer (PH2.5, containing 0.1% HPMC) for the phospholipase A2 electrophoresis buffer system for separation and purification of multi-step purification of calmodulin and phospholipase A2 purity analysis, at the same time for further study of calmodulin and The biological activity of phospholipase A2 provides an effective means of detection.