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目的对鼠源大肠癌细胞肝转移动物模型的建立方法进行改良,为研究大肠癌肝转移机制及生物学行为提供稳定平台。方法选取鼠大肠癌细胞株CT26经皮下传3代后制成细胞悬液与CT26细胞悬液分别接种于BALB/c小鼠脾脏内,实验组为经皮下传3代后脾脏接种,对照组为CT26细胞悬液直接脾脏接种。5 min后切除脾脏。观察其成瘤情况、肝脏转移率、淋巴转移率、肺转移率及生存期,并对2种模型进行初步比较。结果术后第12天实验组肝脏转移率(75.0%)高于对照组(28.6%)(P<0.05);术后第15天及21天后实验组肝脏转移率(93.7%、100.0%)也明显高于对照组(50.0%、66.7%)(P<0.05),但肺脏转移率及淋巴转移率(12.5%、25.0%;26.7%、53.3%)与对照组(0、6.25%;0%、16.7%)相比无明显差异(P>0.05)。实验组平均生存期为(23.56±3.17)d,对照组为(28.14±4.19)d。病理结果证实肝转移肿瘤符合典型的低分化腺癌特征。结论 CT26细胞在免疫功能完整的BALB/c小鼠体内,仍具有良好的成瘤性和转移率,并且经传代后的悬液成瘤时间更短,转移率更高。
Objective To improve the establishment of animal models of hepatic metastasis of murine colon cancer cells and provide a stable platform for studying the mechanism and biological behavior of liver metastasis of colorectal cancer. METHODS: Murine colon cancer cell line CT26 was subcutaneously transduced for 3 generations and then made into cell suspension and CT26 cell suspension were inoculated into the spleen of BALB/c mice. The experimental group was inoculated with the spleen after 3 generations of subcutaneous transfusion. The control group was The CT26 cell suspension was inoculated directly to the spleen. The spleen was removed 5 minutes later. The tumor formation, liver metastasis, lymphatic metastasis rate, lung metastasis rate and survival period were observed, and the two models were compared. Results On the 12th day after operation, the liver metastasis rate in the experimental group was higher (75.0%) than that in the control group (28.6%) (P<0.05). The liver metastasis rate (93.7%, 100.0%) was also observed in the experimental group on the 15th and 21st days after operation. Significantly higher than the control group (50.0%, 66.7%) (P <0.05), but the lung metastasis rate and lymph node metastasis rate (12.5%, 25.0%; 26.7%, 53.3%) and control group (0, 6.25%; 0% There was no significant difference between the two groups (16.7%) (P>0.05). The average survival time was (23.56±3.17) days in the experimental group and (28.14±4.19) days in the control group. Pathological results confirmed that hepatic metastases corresponded with typical poorly differentiated adenocarcinoma features. Conclusion CT26 cells still have good tumorigenicity and metastasis rate in BALB/c mice with intact immune function. After passage, the tumor formation time is shorter and the metastasis rate is higher.