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本文介绍了我们实验室在酶活性不可逆改变动力学理论研究方面所取得的结果。根据所导出的动力学方程,竞争性、非竞争性和反竞争性的概念,同样可用于不可逆抑制作用,并建立了区别这3种不可逆抑制类型以及络合型和非络合型抑制剂的定量判据和计算动力学参数的方法。它不仅适用于不可逆抑制动力学的研究,而且也可用于酶的慢可逆抑制和激活动力学的研究以及酶分子伸展、再卷曲和失活、复活动力学研究。它较之传统动力学方法的主要优点在于:(1)在酶、底物和抑制剂(或激活剂)共存的条件下由一次实验即可求得酶和抑制剂(或激活剂)结合的表观速度常数;(2)所得到的结果更接近生物体内的实际情况;(3)可用于测定快反应过程。这一动力学方法目前已为国际上的同行广泛引证和应用。
This article presents the results of our lab on the kinetic theory of irreversible changes in enzyme activity. According to the derived kinetic equations, the notion of competitive, non-competitive and anti-competitive can also be used for irreversible inhibition, and a distinction is drawn between the three types of irreversible inhibition as well as the complexation and non-complexation inhibitors Quantitative criteria and methods to calculate kinetic parameters. It is not only applicable to irreversible inhibition of kinetics, but also can be used for the study of slow reversible inhibition and activation kinetics of enzymes and molecular stretch, re-curling and inactivation, and resurrection kinetics. The main advantages over traditional kinetic methods are: (1) An enzyme and inhibitor (or activator) binding can be determined in one experiment under the coexistence of enzyme, substrate and inhibitor (or activator) Apparent velocity constant; (2) The results obtained are closer to the actual situation in vivo; (3) Can be used to determine the rapid reaction process. This method of dynamics has now been widely cited and applied internationally.