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目的探讨异体抗CD3单抗激活的杀伤细胞(CD3AK)对严重联合免疫缺陷(SCID)小鼠人肾癌移植瘤的生长抑制作用。方法建立人肾癌SCID鼠皮下移植瘤模型,尾静脉注射异体CD3AK细胞,观察小鼠的生物学特征。SCID小鼠随机分为4组,A组(荷瘤前尾静脉注射CD3AK细胞),B组(荷瘤前尾静脉注射PBS),C组(荷瘤后尾静脉注射CD3AK细胞),D组(荷瘤后尾静脉注射PBS),每组8只。免疫组化检测CD3+细胞在小鼠肿瘤组织中的表达;测量小鼠体质量、肿瘤体积;计算脾脏指数(脾质量/体质量),观察异体CD3AK细胞对肾肿瘤生长的抑制作用及其对小鼠免疫功能的作用。结果 A、C组与B、D组比较,其脾脏指数显著增大,肿瘤质量显著减小,肿瘤生长缓慢(P<0.05)。A组与C组比较脾脏指数无明显差异(P>0.05),A组肿瘤质量减小,肿瘤生长迟缓(P<0.05)。免疫组化示,CD3+细胞在C组肿瘤组织中有阳性表达。结论异体CD3AK细胞对人肾癌SCID鼠移植瘤具有显著抑制作用,且能增强小鼠机体免疫功能,发挥其抗肿瘤作用。
Objective To investigate the inhibitory effect of anti-CD3 monoclonal antibody-activated killer cells (CD3AK) on the growth of human renal carcinoma xenografts in mice with severe combined immunodeficiency (SCID). Methods The subcutaneous xenograft models of human renal carcinoma SCID mice were established. Allogeneic CD3AK cells were injected into tail vein to observe the biological characteristics of mice. SCID mice were randomly divided into 4 groups: group A (CD3AK cells injected into the tail vein before tumor-bearing), group B (PBS injected into the tail vein before tumor-bearing), group C (CD3AK cells injected into the tail vein after tumor- After the tumor-bearing tail vein injection of PBS), each group of eight. Immunohistochemistry was used to detect the expression of CD3 + cells in mouse tumor tissues. The body weight and tumor volume of mice were measured. The spleen index (spleen mass / body weight) was calculated and the inhibitory effect of allogeneic CD3AK cells on the growth of renal tumors was observed. Mouse immune function. Results A, C group compared with B, D group, the spleen index was significantly increased, the tumor mass was significantly reduced, the tumor growth was slow (P <0.05). There was no significant difference in spleen index between group A and group C (P> 0.05). The tumor mass of group A was decreased and the growth of tumor was slow (P <0.05). Immunohistochemistry showed that CD3 + cells were positively expressed in the tumor tissue of group C. Conclusion Allogeneic CD3AK cells have a significant inhibitory effect on human renal carcinoma SCID mice transplanted tumor, and can enhance the body’s immune function and play its anti-tumor effect.